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What is PRP?




Platelet Growth Factor Stimulates Stem Cell Production
PRP (Platelet rich plasma) is defined as autologous blood with a concentration of platelets above baseline values.
• Average normal platelet count = 200,000/ul (normal range: 150,000 – 350,000/ul)
• PRP count= 1,000,000/ul (5x concentration)

Blood is made up of 4 components including plasma, red blood cell (RBC), white blood cell (WBC), and platelets.

A normal blood would be made up of 93% red blood cell, 6% platelets and 1% white blood cell. Through the use of

centrifuge, the blood components are separated and the ratios are changed in PRP to 94% platelets and 5% red blood cell.


Platelets contain an abundance of growth factors which play a central role
in blood clotting and initiate tissue healing including;


• Increase tissue regeneration
• Decrease pain
• Increase collagen (foundational component of all soft tissue)
• Increase bone density
• Increase angiogenesis (development of new blood cells)

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Growth Factor Function

Transforming Growth Factor-beta

• Stimulates undifferentiated mesenchymal cell proliferation
• Regulates endothelial, fibroblastic and osteoblastic mitogenesis
Regulates collagen synthesis and collagenase secretion
• Regulates mitogenic effects of other growth factors
• Stimulates endothelial chemotaxis and angiogenesis
• Inhibits macrophage and lymphocyte proliferation

Platelet Derived Growth Factor

• Mitogenetic for mesenchymal cells and osteoblasts
• Stimulates chemotaxis and mitogenesis in fibroblasts/ smooth muscle cells
• Regulates collagenase secretion and collagen synthesis
• Stimulates macrophage and neutrophil chemotaxis

Epidermal Growth Factor

• Stimulates endothelial chemotaxis and angiogenesis
• Regulates collagenase secretion
Stimulates epithelial and mesenchymal mitogenesis

Vascular Endothelial Growth Factor

• Increases angiogenesis and vessel permeability
• Stimulates mitogenesis for endothelial cells

Basic Fibroblast Growth Factor

• Promotes growth and differentiation of chondrocytes and osteoblasts
• Mitogenetic for mesenchymal cells, chondrocytes and osteoblasts

Connective Tissue Growth Factor

• Promotes angiogenesis and cartilage regeneration
• Promotes fibrosis and platelet adhesion


To prepare PRP, a small amount of blood is taken from the patient. The blood is then placed in a centrifuge.

The centrifuge spins and through a multi-functional process separates the plasma from the blood producing the PRP.

This increases the concentration of platelets and human stem cells proliferation.

When PRP is injected into the damaged area, it triggers the healing cascade as it reduces overall time of inflammation phase.


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